Difference between revisions of "PucH"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''pucH'' {{PubMed|12029039}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12029039}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
+
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [PucR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
+
** [[PucR]]: transcription activation {{PubMed|12029039}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
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=References=
 
=References=
  
<pubmed>11344136,,12029039,12029039, </pubmed>
+
<pubmed>11344136,,12029039, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:04, 28 December 2009

  • Description: allantoinase

Gene name pucH
Synonyms yunH
Essential no
Product allantoinase
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 48 kDa, 5.062
Gene length, protein length 1338 bp, 446 aa
Immediate neighbours yunG, pucR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PucH context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32410

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-allantoin + H2O = allantoate (according to Swiss-Prot)
  • Protein family: Allantoinase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): PyrC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)