pucH

168

allantoinase

locus: BSU_32410

Molecular weight: 48.17 kDa

pI: 5.06

Protein length: 446 aa

Gene length: 1341 bp

function: purine utilization

product: allantoinase

essential: no

ec: 3.5.2.5

synonyms: pucH, yunH

List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)

Gene

Coordinates: 3,327,247  3,328,587

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The protein

Catalyzed reaction/ biological activity

• (S)-allantoin + H₂O --> allantoate + H⁺ (according to UniProt)

Protein family

• [wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|3HM7] (from ''Bacillus halodurans'', 44% identity, 65% similarity)
• [AF|O32137]

Paralogous protein(s)

• [protein|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]

Biological materials

Mutant

• MGNA-A933 (yunH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/933 NBRP B. subtilis, Japan]
• BKE32410 ([gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCCCCCCATGTC,  downstream forward: _UP4_TAAGCCCGGCGGATTTTACC
• BKK32410 ([gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCCCCCCATGTC,  downstream forward: _UP4_TAAGCCCGGCGGATTTTACC

References

• 11344136,12029039