pucG

168

(S)-ureidoglycine-glyoxylate aminotransferase

locus: BSU_32520

Molecular weight: 45.58 kDa

pI: 5.42

Protein length: 416 aa

Gene length: 1251 bp

function: purine utilization

product: (S)-ureidoglycine-glyoxylate aminotransferase

essential: no

synonyms: pucG, yurG

List of homologs in different organisms, belongs to COG0075 (Galperin et al., 2021)

Gene

Coordinates: 3,341,166  3,342,416

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The protein

Catalyzed reaction/ biological activity

• transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine [Pubmed|20852637]
• (S)-2-ureidoglycine + glyoxylate --> glycine + N-carbamoyl-2-oxoglycine (according to UniProt)

Protein family

• [wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|3ISL]\t[Pubmed|20852637]
• [AF|O32148]

Biological materials

Mutant

• MGNA-A942 (yurG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/942 NBRP B. subtilis, Japan]
• BKE32520 ([gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCCTGACACAGCCATTCCTC,  downstream forward: _UP4_TAAAGAAAAGCTTGCGGAAC
• BKK32520 ([gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCCTGACACAGCCATTCCTC,  downstream forward: _UP4_TAAAGAAAAGCTTGCGGAAC

References

• 11344136,20852637,12029039