PucF

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  • Description: allantoate amidohydrolase

Gene name pucF
Synonyms yurH
Essential no
Product allantoate amidohydrolase
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 45 kDa, 5.364
Gene length, protein length 1236 bp, 412 aa
Immediate neighbours pucG, yurI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YurH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PucF expression.png




























Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon

The gene

Basic information

  • Locus tag: BSU32530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase M20 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2IMO (from Escherichia coli k12, 47% identity, 63% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References