pucF

pucF
168

allantoate amidohydrolase

locus
BSU_32530
Molecular weight
45.36 kDa
pI
5.36
Protein length
Gene length
function
purine utilization
product
allantoate amidohydrolase
essential
no
synonyms
pucF, yurH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0624 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,342,433 → 3,343,671
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The protein
Catalyzed reaction/ biological activity
allantoate + 2 H+ + H2O --> (S)-2-ureidoglycine + CO2 + NH4+ (according to UniProt)
Protein family
[wiki|peptidase M20 family] (according to UniProt)
Structure
[PDB|2IMO] (from ''Escherichia coli k12'', 47% identity, 63% similarity) [Pubmed|17362992]
[AF|O32149]
Expression and Regulation
Operons
genes
[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]-[gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]
description
[Pubmed|12029039]
regulation
induced in the presence of purine nucleotides (inducer: allantoin) ([protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]) [Pubmed|12029039]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]→[gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]

2024-03-19 20:20:18

Jstuelk

69

8950B371B49B2D867C39C0645E288618F9712782

C7BE4A3C8762E1DBFB97C41D65DC9339DE1A1137

Biological materials
Mutant
MGNA-A943 (yurH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/943 NBRP B. subtilis, Japan]
BKE32530 (Δ[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC,  downstream forward: _UP4_GCTTACTGATAAAGGAGGAA
BKK32530 (Δ[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC,  downstream forward: _UP4_GCTTACTGATAAAGGAGGAA
References
11344136,12029039,35171018

C314CE9D194E8894430368C5F69C0778D45A651C

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Time of last update: 2024-03-28 11:57:15

Author of last update: Jstuelk