pucE

168

xanthine dehydrogenase

locus: BSU_32470

Molecular weight: 18.69 kDa

pI: 6.13

Protein length: 173 aa

Gene length: 522 bp

function: purine utilization

product: xanthine dehydrogenase

essential: no

synonyms: pucE, yurB

List of homologs in different organisms, belongs to COG2080 (Galperin et al., 2021)

Gene

Coordinates: 3,335,751  3,336,272

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The protein

Catalyzed reaction/ biological activity

• H₂O + NAD⁺ + xanthine --> H⁺ + NADH + urate (according to UniProt)
• H₂O + hypoxanthine + NAD⁺ --> H⁺ + NADH + xanthine (according to UniProt)

[wiki|Domains]

• 2Fe-2S ferredoxin-type domain (aa 14-90) (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|1JRO] (from ''Rhodobacter capsulatus'', 43% identity, 58% similarity) [Pubmed|11796116]
• [AF|O32143]

Biological materials

Mutant

• MGNA-A939 (yurB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/939 NBRP B. subtilis, Japan]
• BKE32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT,  downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
• BKK32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT,  downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG

References

• 11344136,12029039,12823818