pucE

pucE
168

xanthine dehydrogenase

locus
BSU_32470
Molecular weight
18.69 kDa
pI
6.13
Protein length
Gene length
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
pucE, yurB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2080 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,335,751  3,336,272
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The protein
Catalyzed reaction/ biological activity
H2O + NAD+ + xanthine --> H+ + NADH + urate (according to UniProt)
H2O + hypoxanthine + NAD+ --> H+ + NADH + xanthine (according to UniProt)
[wiki|Domains]
2Fe-2S ferredoxin-type domain (aa 14-90) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|1JRO] (from ''Rhodobacter capsulatus'', 43% identity, 58% similarity) [Pubmed|11796116]
[AF|O32143]
Expression and Regulation
Operons
genes
[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
description
[Pubmed|12823818]
regulation
induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]→[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]

2024-03-28 03:59:35

ghost

60

12efbe45279270e4577ccc311231d5d18e3f41c8

DEA144E8D38829EB38B1ABABEC21C2CCD65FEFF4

Biological materials
Mutant
MGNA-A939 (yurB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/939 NBRP B. subtilis, Japan]
BKE32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT,  downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
BKK32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT,  downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
References
11344136,12029039,12823818

6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843

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Time of last update: 2024-03-28 12:03:25

Author of last update: Melvin.boenninger