pucA

168

xanthine dehydrogenase

locus: BSU_32510

Molecular weight: 36.59 kDa

pI: 6.36

Protein length: 330 aa

Gene length: 993 bp

function: purine utilization

product: xanthine dehydrogenase

essential: no

synonyms: pucA, yurF

List of homologs in different organisms, belongs to COG1975 (Galperin et al., 2021)

Gene

Coordinates: 3,339,945 → 3,340,937

Advertisement

The protein

Catalyzed reaction/ biological activity

• H₂O + NAD⁺ + xanthine --> H⁺ + NADH + urate (according to UniProt)
• H₂O + hypoxanthine + NAD⁺ --> H⁺ + NADH + xanthine (according to UniProt)

Structure

• [PDB|3ON5] (from B. halodurans, 44% identity)
• [AF|O32147]

Biological materials

Mutant

• MGNA-A965 (yurF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/965 NBRP B. subtilis, Japan]
• BKE32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC,  downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT
• BKK32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC,  downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT

References

• 11344136,12029039,12823818