pucA

pucA
168

xanthine dehydrogenase

locus
BSU_32510
Molecular weight
36.59 kDa
pI
6.36
Protein length
Gene length
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
pucA, yurF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1975 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,339,945 → 3,340,937
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The protein
Catalyzed reaction/ biological activity
H2O + NAD+ + xanthine --> H+ + NADH + urate (according to UniProt)
H2O + hypoxanthine + NAD+ --> H+ + NADH + xanthine (according to UniProt)
Structure
[PDB|3ON5] (from B. halodurans, 44% identity)
[AF|O32147]
Expression and Regulation
Operons
genes
[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
description
[Pubmed|12823818]
regulation
induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]→[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]

2024-03-28 03:59:35

ghost

60

12efbe45279270e4577ccc311231d5d18e3f41c8

DEA144E8D38829EB38B1ABABEC21C2CCD65FEFF4

Biological materials
Mutant
MGNA-A965 (yurF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/965 NBRP B. subtilis, Japan]
BKE32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC,  downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT
BKK32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC,  downstream forward: _UP4_ACAAGAAAACGGGTGGCGGT
References
11344136,12029039,12823818

C9370B221117B89A1134B413748D39505C2E1DA8

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Time of last update: 2024-03-28 19:28:29

Author of last update: Melvin.boenninger