Difference between revisions of "PucA"

From SubtiWiki
Jump to: navigation, search
Line 89: Line 89:
  
 
=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[pucA]]-[[pucB]]-[[pucC]]-[[pucD]]-[[pucE]]'' {{PubMed|12823818}}
  
* '''Operon:'''
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12029039}}
 
 
* '''[[Sigma factor]]:'''  
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed in the absence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
+
** repressed by purine nucleotides (effector: allantoin) ([[PucR]]) {{PubMed|12029039}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [PucR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
+
** [[PucR]]: transcription repression {{PubMed|12029039}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
Line 122: Line 121:
 
=References=
 
=References=
  
<pubmed>11344136,,12029039,12029039, </pubmed>
+
<pubmed>11344136,,12029039,12823818, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:37, 28 December 2009

  • Description: xanthine dehydrogenase

Gene name pucA
Synonyms yurF
Essential no
Product xanthine dehydrogenase
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Purine catabolism, Nucleotides (regulation)
MW, pI 36 kDa, 6.359
Gene length, protein length 990 bp, 330 aa
Immediate neighbours pucB, pucG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PucA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by purine nucleotides (effector: allantoin) (PucR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)