ptsI

168

Enzyme I, general (non sugar-specific) component of the PTS

locus: BSU_13910

Molecular weight: 62.90 kDa

pI: 4.59

Protein length: 570 aa

Gene length: 1713 bp

function: PTS-dependent sugar transport

product: phosphotransferase system (PTS) enzyme I

essential: no

ec: 2.7.3.9

synonyms: ptsI

List of homologs in different organisms, belongs to COG1080 (Galperin et al., 2021)

Gene

Coordinates: 1,459,650 1,461,362

Phenotypes of a mutant

• poorly transformable [pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• L-histidyl-[protein] + phosphoenolpyruvate --> N-phospho-L-histidyl-[protein] + pyruvate (according to UniProt)
• PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr]]] (His-15)

Protein family

• [wiki|PEP-utilizing enzyme family] (according to UniProt)

[wiki|Domains]

• HPr binding site (N-Terminal Domain)
• pyruvate binding site (C-Terminal Domain)
• pyrophosphate/phosphate carrier histidine (central Domain)

[wiki|Cofactors]

• Magnesium

Structure

• [PDB|2WQD] (Enzyme I from ''Staphylococcus aureus'', 68% identity) [Pubmed|19801641]
• [AF|P08838]

Modification

• transient autophosphorylation on His-189
• ''in vivo'' also phosphorylated on Ser-34 or Ser-36 [Pubmed|17218307]

[wiki|Localization]

• cytoplasm, even distribution [Pubmed|23475962]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• available in [wiki|Jörg Stülke]'s lab:
• GP864 ([gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::ermC)
• GP778 ([gene|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|glcT]-[gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]-[gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]-[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::spc) [Pubmed|22722928]
• BKE13910 ([gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCAATCCCTTTTAATTCTT, downstream forward: _UP4_TAATGTACAAAAACCAGACG
• BKK13910 ([gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACCAATCCCTTTTAATTCTT, downstream forward: _UP4_TAATGTACAAAAACCAGACG

Expression vectors

• pAG3 (His-tag) [Pubmed|9237995], available in [wiki|Galinier] lab
• for expression, purification in ''E. coli'' (His-tag), in [wiki|pWH844]: pGP813 available in [wiki|Jörg Stülke]'s lab

GFP fusion

• GP1268, [gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]-cfp, available in [wiki|Jörg Stülke]'s lab [pubmed|23475962]

labs

• [wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

References

Reviews

• 24847021,17158705,11018147,9871918,18628769

Original Publications

• 12850135,17218307,9721285,20081037,19801641,23475962,24129255,15378759,28189581,36815589