ptpZ

168

phosphotyrosine protein phosphatase, antagonist to [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]

locus: BSU_36240

Molecular weight: 28.81 kDa

pI: 6.66

Protein length: 254 aa

Gene length: 765 bp

function: protein tyrosine dephosphorylation

product: phosphotyrosine protein phosphatase, antagonist to [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]

essential: no

ec: 3.1.3.48

synonyms: ptpZ, ywqE

List of homologs in different organisms, belongs to COG4464 (Galperin et al., 2021)

Gene

Coordinates: 3,731,005 → 3,731,769

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The protein

Catalyzed reaction/ biological activity

• H₂O + O-phospho-L-tyrosyl-[protein] --> L-tyrosyl-[protein] + phosphate (according to UniProt)
• dephosphorylation of [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd], [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD] and [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA] [Pubmed|15866923]

Protein family

• [wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|3QY7] [Pubmed|21605684]
• [AF|P96717]

Biological materials

Mutant

• MGNA-A077 (ywqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/77 NBRP B. subtilis, Japan]
• GP1609 (spc), available in [wiki| Jörg Stülke]'s lab
• GP1610 (''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]'', spc), available in [wiki| Jörg Stülke]'s lab
• BKE36240 (Δ[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTAGCCCCCTTTTT,  downstream forward: _UP4_TAACAGCCGATTCTCATTTC
• BKK36240 (Δ[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTAGCCCCCTTTTT,  downstream forward: _UP4_TAACAGCCGATTCTCATTTC

References

• 15866923,12970183,21605684,20815827,21605684,20817675,26283769,27148221