ptb

168

phosphate butyryltransferase

locus: BSU_24090

Molecular weight: 31.62 kDa

pI: 7.94

Protein length: 299 aa

Gene length: 900 bp

function: utilization of branched-chain keto acids

product: phosphate butyryltransferase

essential: no

ec: 2.3.1.19

synonyms: ptb, yqiS, bkd

List of homologs in different organisms, belongs to COG0280 (Galperin et al., 2021)

Gene

Coordinates: 2,503,765  2,504,664

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The protein

Catalyzed reaction/ biological activity

• butanoyl-CoA + phosphate --> butanoyl phosphate + CoA (according to UniProt)

Protein family

• phosphate acetyltransferase and butyryltransferase family (with [protein|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta], according to UniProt)

Structure

• [PDB|1YCO] (from ''Enterococcus faecalis v583'', 36% identity, 56% similarity)
• [AF|P54530]

Biological materials

Mutant

• MGNA-C417 (yqiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2415 NBRP B. subtilis, Japan]
• BKE24090 ([gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTACCACCTTTCTT,  downstream forward: _UP4_TACACACATTAGGAGGAACG
• BKK24090 ([gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24090 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTACCACCTTTCTT,  downstream forward: _UP4_TACACACATTAGGAGGAACG

References

• 12427936,15241682,12823818,10094682,28165735