PstS

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Gene name pstS
Synonyms yqgG, yzmB
Essential no
Product phosphate ABC transporter (binding protein)
Function high-affinity phosphate uptake
Gene expression levels in SubtiExpress: pstS
Interactions involving this protein in SubtInteract: PstS
MW, pI 31 kDa, 4.845
Gene length, protein length 900 bp, 300 aa
Immediate neighbours pstC, pbpA
Sequences Protein DNA Advanced_DNA
Genetic context
PstS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PstS expression.png
























Categories containing this gene/protein

ABC transporters, phosphate metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

  • Locus tag: BSU24990

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: pstS family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cell membrane (according to Swiss-Prot)
    • extracellular (signal peptide) PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

Your additional remarks

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

Nicholas E E Allenby, Nicola O'Connor, Zoltán Prágai, Noel M Carter, Marcus Miethke, Susanne Engelmann, Michael Hecker, Anil Wipat, Alan C Ward, Colin R Harwood
Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter.
Microbiology (Reading): 2004, 150(Pt 8);2619-2628
[PubMed:15289558] [WorldCat.org] [DOI] (P p)

Akram Atalla, Wolfgang Schumann
The pst operon of Bacillus subtilis is specifically induced by alkali stress.
J Bacteriol: 2003, 185(16);5019-22
[PubMed:12897025] [WorldCat.org] [DOI] (P p)

H Antelmann, C Scharf, M Hecker
Phosphate starvation-inducible proteins of Bacillus subtilis: proteomics and transcriptional analysis.
J Bacteriol: 2000, 182(16);4478-90
[PubMed:10913081] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

W Liu, Y Qi, F M Hulett
Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity.
Mol Microbiol: 1998, 28(1);119-30
[PubMed:9593301] [WorldCat.org] [DOI] (P p)

Y Qi, Y Kobayashi, F M Hulett
The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.
J Bacteriol: 1997, 179(8);2534-9
[PubMed:9098050] [WorldCat.org] [DOI] (P p)