Difference between revisions of "PstA"

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(Expression and regulation)
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
 
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
  
* '''Regulation:''' expressed under conditions of phosphate limitation (5000-fold induced) ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
+
* '''Regulation:''' expressed under conditions of phosphate limitation (5000-fold induced) ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]
** expressed under conditions of phosphate limitation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]
 
  
* '''Regulatory mechanism:''' [[PhoP]]-P: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050 PubMed]
+
* '''Regulatory mechanism:'''  
 
** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]
 
** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 21:14, 20 November 2009

  • Description: phosphate ABC transporter (permease)

Gene name pstA
Synonyms yqgI, yzmD
Essential no
Product phosphate ABC transporter (permease)
Function high-affinity phosphate uptake
MW, pI 31 kDa, 9.303
Gene length, protein length 882 bp, 294 aa
Immediate neighbours pstBA, pstC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PstA context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU24970

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: CysTW subfamily (according to Swiss-Prot)
  • Paralogous protein(s): PstC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

Your additional remarks

References

Nicholas E E Allenby, Nicola O'Connor, Zoltán Prágai, Noel M Carter, Marcus Miethke, Susanne Engelmann, Michael Hecker, Anil Wipat, Alan C Ward, Colin R Harwood
Post-transcriptional regulation of the Bacillus subtilis pst operon encoding a phosphate-specific ABC transporter.
Microbiology (Reading): 2004, 150(Pt 8);2619-2628
[PubMed:15289558] [WorldCat.org] [DOI] (P p)

Akram Atalla, Wolfgang Schumann
The pst operon of Bacillus subtilis is specifically induced by alkali stress.
J Bacteriol: 2003, 185(16);5019-22
[PubMed:12897025] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

W Liu, Y Qi, F M Hulett
Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity.
Mol Microbiol: 1998, 28(1);119-30
[PubMed:9593301] [WorldCat.org] [DOI] (P p)

Y Qi, Y Kobayashi, F M Hulett
The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.
J Bacteriol: 1997, 179(8);2534-9
[PubMed:9098050] [WorldCat.org] [DOI] (P p)