pspA

168

phage shock protein A homolog, protects the membrane against membrane-targeting antibiotics

locus: BSU_06180

Molecular weight: 24.99 kDa

pI: 5.78

Protein length: 227 aa

Gene length: 684 bp

function: protection of the membrane

product: phage shock protein A homolog

essential: no

synonyms: pspA, ydjF

List of homologs in different organisms, belongs to COG1842 (Galperin et al., 2021)

Gene

Coordinates: 671,245  671,928

Phenotypes of a mutant

• more sensitive to nisin [Pubmed|23980836]

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The protein

Protein family

• PspA/IM30 family (with [protein|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH], according to UniProt)

Structure

• [PDB|4WHE] (from E. coli, 34% identity, corresponds to aa 1 ... 133) [pubmed|26235546]
• [AF|P54617]

Paralogous protein(s)

• [protein|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]

[wiki|Localization]

• cytoplasma (according to UniProt)
• localizes to the membrane under stress conditions (localization depends of [protein|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]) [pubmed|34777311]

Biological materials

Mutant

• MGNA-C430 (yqgI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2428 NBRP B. subtilis, Japan]
• BKE06180 ([gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGTTCCTCCTAA,  downstream forward: _UP4_TAATACATAGGAGGCCGCAG
• BKK06180 ([gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGTTCCTCCTAA,  downstream forward: _UP4_TAATACATAGGAGGCCGCAG

References

Reviews

• 27297125

Original Publications

• 11454200,18840696,22926563,23980836,27791134,26235546,34777311,35604119