Difference between revisions of "PspA"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* more sensitive to nisin {{PubMed|23980836}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Protein family:''' pspA/IM30 family (according to Swiss-Prot)
 
* '''Protein family:''' pspA/IM30 family (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[LiaH]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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=References=
 
=References=
<pubmed> 11454200 18840696 22926563</pubmed>  
+
<pubmed> 11454200 18840696 22926563 23980836</pubmed>  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:43, 30 August 2013

  • Description: phage shock protein A homolog

Gene name pspA
Synonyms ydjF
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: pspA
MW, pI 24 kDa, 5.775
Gene length, protein length 681 bp, 227 aa
Immediate neighbours fruC, ydjG
Sequences Protein DNA DNA_with_flanks
Genetic context
PspA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PspA expression.png















Categories containing this gene/protein

cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

  • Locus tag: BSU06180

Phenotypes of a mutant

  • more sensitive to nisin PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: pspA/IM30 family (according to Swiss-Prot)
  • Paralogous protein(s): LiaH

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by alkaline shock (SigW) PubMed
    • repressed during logarithmic phase (AbrB) PubMed
    • induced by antibiotics interacting with the cytoplasmic membrane PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Anthony W Kingston, Xiaojie Liao, John D Helmann
Contributions of the σ(W) , σ(M) and σ(X) regulons to the lantibiotic resistome of Bacillus subtilis.
Mol Microbiol: 2013, 90(3);502-18
[PubMed:23980836] [WorldCat.org] [DOI] (I p)

Michaela Wenzel, Bastian Kohl, Daniela Münch, Nadja Raatschen, H Bauke Albada, Leendert Hamoen, Nils Metzler-Nolte, Hans-Georg Sahl, Julia E Bandow
Proteomic response of Bacillus subtilis to lantibiotics reflects differences in interaction with the cytoplasmic membrane.
Antimicrob Agents Chemother: 2012, 56(11);5749-57
[PubMed:22926563] [WorldCat.org] [DOI] (I p)

Allison V Banse, Arnaud Chastanet, Lilah Rahn-Lee, Errett C Hobbs, Richard Losick
Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2008, 105(40);15547-52
[PubMed:18840696] [WorldCat.org] [DOI] (I p)

T Wiegert, G Homuth, S Versteeg, W Schumann
Alkaline shock induces the Bacillus subtilis sigma(W) regulon.
Mol Microbiol: 2001, 41(1);59-71
[PubMed:11454200] [WorldCat.org] [DOI] (P p)