Difference between revisions of "PspA"

From SubtiWiki
Jump to: navigation, search
Line 13: Line 13:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU06180 pspA]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06180 pspA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.775   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.775   
Line 21: Line 21:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fruC]]'', ''[[ydjG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fruC]]'', ''[[ydjG]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12437&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06180 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pspA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pspA_context.gif]]

Revision as of 12:16, 13 May 2013

  • Description: phage shock protein A homolog

Gene name pspA
Synonyms ydjF
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: pspA
MW, pI 24 kDa, 5.775
Gene length, protein length 681 bp, 227 aa
Immediate neighbours fruC, ydjG
Sequences Protein DNA Advanced_DNA
Genetic context
PspA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PspA expression.png















Categories containing this gene/protein

cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

AbrB regulon, SigW regulon

The gene

Basic information

  • Locus tag: BSU06180

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: pspA/IM30 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by alkaline shock (SigW) PubMed
    • repressed during logarithmic phase (AbrB) PubMed
    • induced by antibiotics interacting with the cytoplasmic membrane PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michaela Wenzel, Bastian Kohl, Daniela Münch, Nadja Raatschen, H Bauke Albada, Leendert Hamoen, Nils Metzler-Nolte, Hans-Georg Sahl, Julia E Bandow
Proteomic response of Bacillus subtilis to lantibiotics reflects differences in interaction with the cytoplasmic membrane.
Antimicrob Agents Chemother: 2012, 56(11);5749-57
[PubMed:22926563] [WorldCat.org] [DOI] (I p)

Allison V Banse, Arnaud Chastanet, Lilah Rahn-Lee, Errett C Hobbs, Richard Losick
Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2008, 105(40);15547-52
[PubMed:18840696] [WorldCat.org] [DOI] (I p)

T Wiegert, G Homuth, S Versteeg, W Schumann
Alkaline shock induces the Bacillus subtilis sigma(W) regulon.
Mol Microbiol: 2001, 41(1);59-71
[PubMed:11454200] [WorldCat.org] [DOI] (P p)