prs

168

phosphoribosylpyrophosphate synthetase, universally conserved protein

locus: BSU_00510

Molecular weight: 34.71 kDa

pI: 5.89

Protein length: 317 aa

Gene length: 954 bp

function: phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)

product: phosphoribosylpyrophosphate synthetase

essential: yes

ec: 2.7.6.1

synonyms: prs

List of homologs in different organisms, belongs to COG0462 (Galperin et al., 2021)

Gene

Coordinates: 57,745  58,698

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• ATP + D-ribose 5-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + AMP + H⁺ (according to UniProt)

Protein family

• Ribose-phosphate pyrophosphokinase family (single member, according to UniProt)

Structure

• [PDB|1IBS]
• [PDB|1DKR]
• [PDB|1DKU]
• [AF|P14193]

Effectors of protein activity

• subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]

[wiki|Localization]

• cytoplasm (according to UniProt)

Additional information

• [wiki|universally conserved protein]

Biological materials

Expression vectors

• N-terminal Strep-tag, expression in ''E. coli'', in [wiki|pGP172]: pGP2541, available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 28031352

Original Publications

• 2169413,8522540,12837783,12837784,19446032,22083279