prs

prs
168

phosphoribosylpyrophosphate synthetase, universally conserved protein

locus
BSU_00510
Molecular weight
34.71 kDa
pI
5.89
Protein length
Gene length
function
phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
product
phosphoribosylpyrophosphate synthetase
essential
yes
ec
2.7.6.1
synonyms
prs

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0462 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
57,745  58,698
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
ATP + D-ribose 5-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + AMP + H+ (according to UniProt)
Protein family
Ribose-phosphate pyrophosphokinase family (single member, according to UniProt)
Structure
[PDB|1IBS]
[PDB|1DKR]
[PDB|1DKU]
[AF|P14193]
Effectors of protein activity
subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) [Pubmed|22083279]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
[wiki|universally conserved protein]
Expression and Regulation
Operons
genes
[gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
description
[Pubmed|22383849]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|8522540], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]→[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]

2024-01-23 11:04:18

Jstuelk

75

8a2ec596272c7cfb42cb975d3b44431fc04457cf

58FF1AAE3D8324581B04A0D2CE6BF74D3CC7B05C

Biological materials
Expression vectors
N-terminal Strep-tag, expression in ''E. coli'', in [wiki|pGP172]: pGP2541, available in [wiki|Jörg Stülke]'s lab
References
Reviews
28031352
Original Publications
2169413,8522540,12837783,12837784,19446032,22083279

37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF

Page visits: 3075

Time of last update: 2024-03-28 18:56:09

Author of last update: Melvin.boenninger