Program

Monday March 14th 2011

Registration: 12.30 – 13.45

13.50 - 14.00 Jörg Stülke Welcome and Introduction

Session I: Signalling, motility and biofilm formation

Chair: Eugenio Ferrari

• 14:00 – 14:30 Daniel Kearns: invited speaker

• 14:30 – 14:45 Gyanendra P. Dubey, and Sigal Ben-Yehuda (Hebrew University; Jerusalem, Israel)
  • Intercellular nanotubes mediate bacterial communication

• 14:45 – 15:00 Akos T. Kovacs, and Oscar P. Kuipers (University of Groningen, Netherlands)
  • Regulation of architecturally complex colony development in Bacillus subtilis

• 15:00 – 15:15 Noël Molière, Janine Kirstein, and Kürşad Turgay (Freie Universität Berlin, Germany; and Northwestern University, Evanston, IL USA)
  • Involvement of Hsp100/Clp proteases in the motility development of Bacillus subtilis

• 15:15 – 15:30 Sophie Bochmann, Benedikt Sattler, Sebastian W. Fuchs, Peter Kötter, and Karl-Dieter Entian (Goethe University, Frankfurt am Main, Germany)
  • Analysis of lantibiotic immunity and regulation in Bacillus subtilis

• 15:30 – 15:45 Anna Staroń, Sebastian Dintner, Georg Fritz, Ulrich Gerland, Susanne Gebhard, and Thorsten Mascher (Ludwig-Maximilians-Universität München, Germany)
  • Peptide Antibiotic Sensing and Detoxification Modules of Bacillus subtilis: Stimulus Specificity, Signalling Interfaces and Regulatory Dynamics

• 15:45 – 16:15 Coffee Break

Session II: Localisation and secretion

Chair: Thomas Wiegert

• 16:15 – 16:30 Frank Bürmann, Nina Ebert, Suey van Baarle, and Marc Bramkamp (University of Cologne, Germany)
  • A bacterial dynamin-like protein mediating nucleotide independent membrane fusion

• 16:30 – 16:45 Daniel Lopez (Institute for Molecular Infectious Diseases, Würzburg, Germany)
  • Targeting Lipid Rafts in Bacteria

• 16:45 – 17:00 Tjeerd van Rij, Isabelle Maillet, Thomas Lapointe, Marcel Hillebrand, Sabrina Rodriguez, Jan Maarten van Dijl, and Zoltán Prágai (DSM Biotechnology Center, Delft, the Netherlands; DSM Nutritional Products, Basel, Switzerland; and UMCG-University of Groningen, the Netherlands)
  • Secretome of the industrial Bacillus amyloliquefaciens strain BZ9

• 17:00 – 17:15 Simon Stammen, Claudia Korneli, Britta K. Müller, Tobias Knuuti, Constanze Finger, Ezequiel Franco-Lara, Dieter Jahn, and Rebekka Biedendieck (Technische Universität Braunschweig, Germany)
  • High-yield intra- and extracellular protein production using Bacillus megaterium

• 17:15 – 17:30 Krzysztof Gizynski, Susanne Pohl and Colin R. Harwood (Newcastle University, Newcastle upon Tyne, United Kingdom)
  • The secondary protein secretion translocase of Bacillus anthracis

Session III: Replication–Cell cycle

Chair: Thorsten Mascher

• 17:30 – 17:45 V. Bidnenko, S. McGovern, F. Lecointe, P. Noirot, and M.-F. Noirot-Gros (INRA, Jouy en Josas, France)
  • The B. subtilis negative controller of replication initiation YabA acts as a replication checkpoint

• 17.45 – 18.00 Hamid Nouri, Anne-Françoise Monnier, Olivier Rannou, Monika Maciag, Solveig Fossum, Armelle Cabin-Flaman, Gregorz Wegrzyn, Vic Norris, Agnieszka Szalewska-Palasz, Panos Soultanas, Kirsten Skarstad, and Laurent Jannière (INRA, Jouy en Josas, France; Génopole Campus I, Evry, France; University of Nottingham, UK; University of Gdansk, Poland; Rikshospitalet, University of Oslo, Norway; and Faculté des Sciences de Rouen, Mont-Saint-Aignan, France)
  • Does glycolysis gate DNA replication in the Bacillus subtilis cell cycle?

• 18.00 – 18.15 Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Rault-Ventroux, Alain Trubuil, Marie-Françoise Noirot-Gros, and Ivan Mijakovic (INRA- AgroParisTech, and INRA, Jouy-en-Josas, France)
  • Phosphorylated bacterial recombinase checks chromosome integrity during spore formation

• 18.15 – 18.30 Imrich Barák, Naďa Pavlendová, Ján Jamoroškovič, Anthony J. Wilkinson, and Katarína Muchová (Slovak Academy of Sciences, Bratislava, Slovakia; and University of York, UK)
  • Min system oscillation is adverse for asymmetric cell division during Bacillus subtilis sporulation

Tuesday March 15th 2011

• 8.40 – 8.45 Jörg Stülke

Welcome to session: RNA biology of Bacillus

Session IV: RNA biology of Bacillus

Chair: Tony Wilkinson

• 8.45 – 9.00 Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, the BaSysBio consortium, Michael Hecker, Uwe Völker, Philippe Bessières, and Philippe Noirot (INRA, Jouy-en-Josas, France; Ernst-Moritz-Arndt-Universität Greifswald, Germany; and INRA-AgroParisTech, Jouy-en-Josas, France)
  • Quantitative and qualitative analysis of the Bacillus subtilis whole-transcriptome across lifestyles

• 9.00 – 9.15 Marc Schaffer, Anja Wiechert, Hanna Daniel, Claudia Schurmann, Georg Homuth, Michael Hecker, Volkmar Liebscher, and Ulrike Mäder (Ernst- Moritz-Arndt-University Greifswald, Germany)
  • Genome-wide analysis of mRNA half-lives in Bacillus subtilis

• 9.15 - 9.30 Ruben A. T. Mars, Emma L. Denham, Pierre Nicolas, Philippe Noirot, Ulrike Mäder, Jan Maarten van Dijl and the BaSysBio and BACELL SysMo consortia (UMCG-University of Groningen, the Netherlands; INRA, Jouy-en-Josas, France; INRA-AgroParisTech, Jouy-en-Josas , France; and Ernst-Moritz-Arndt-University Greifswald, Germany)
  • The non-coding RNAs of Bacillus subtilis

• 9.30 – 9.45 Matthias Gimpel, Heike Preis, Natalie Jahn, Nadja Heidrich, Andreas Licht, Ulrike Mäder, and Sabine Brantl (Friedrich-Schiller-Universität, Jena, Germany; Universität Würzburg, Germany; Jena Bioscience GmbH, Jena, Germany; Ernst-Moritz-Arndt-Universität Greifswald, Germany)
  • Two sRNA regulated systems from the Bacillus subtilis chromosome

• 9.45 – 10.00 Martin Lehnik, Christina Herzberg, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, and Jörg Stülke (Georg-August-Universität Göttingen, Germany; Ernst-Moritz-Arndt-University Greifswald, Germany)
  • Structural and functional association of RNases in Bacillus subtilis

• 10.00 – 10.15 Karen Shahbabian, Ailar Jamalli, Léna Zig, and Harald Putzer (CNRS Paris, France)
  • RNase Y and RNase J, the two major players in mRNA metabolism in Bacillus subtilis

• 10.15 – 10.30 Joseph A. Newman, Lorraine Hewitt, Cecilia Rodrigues, Alexandra Solovyova, Colin R. Harwood, and Richard J. Lewis (Newcastle University, Newcastle-upon-Tyne, U.K.)
  • Unusual, dual endo- and exo-nuclease activity in the degradosome explained by crystal structure analysis of RNase J1

• 10.30 – 11.00 Coffee/Tea

Session IV; RNA biology of Bacillus; continued

Chair: Ulrike Mäder

11.00 – 11.15 Katharina Schäfer, Ulrike Mäder, Georg Homuth, and Thomas Wiegert (University of Bayreuth, Germany; Ernst-Moritz-Arndt-University Greifswald, Germany; and University of Applied Sciences, Zittau/Görlitz, Germany)

• • Analysis of Rho-dependent termination of the Bacillus subtilis sigX-rsiX operon

11.15 – 11.30 Anne R. Karow, Regula Aregger, and Dagmar Klostermeier (University of Basel, Biozentrum, Switzerland; and University of Muenster, Germany

• • Dissecting the mechanism of the DEAD box protein YxiN from Bacillus subtilis

11.30 – 11.45 Hermann Hämmerle, Branislav Večerek, Ivo Hofacker, and Udo Bläsi (Max F. Perutz Laboratories, University of Vienna, Austria; Czech Academy of Sciences, Prague, Czech Republic; and Institute for Theoretical Chemistry, University of Vienna, Austria)

• • Identification of candidate Hfq targets in Bacillus subtilis

11.45 – 12.00 Sandra Wiegand, Sascha Dietrich, and Heiko Liesegang (Georg-August-University of Göttingen, Germany)

• • Regulatory elements of Bacillus licheniformis DSM 13

Session V: Tools Chair: Ulrike Mäder (continued) 12.00 – 12.15 Jan Muntel, Vincent Fromion, Michael Hecker and Dörte Becher (Ernst-Moritz-Arndt-University of Greifswald, Germany; and INRA, Jouy-en-Josas, France)

• • New approach for global absolute protein quantification of B. subtilis

12.15 – 12.30 Valérie Leclère, Maude Pupin; Walaa Hussein, Max Béchet, Frédérique Gancel, Marlène Chollet, Ségolène Caboche, and Philippe Jacques (Université de Lille Nord de France, Sciences et Technologies, Villeneuve d'Ascq, France)

• • Bioinformatics tools to decrypt non-ribosomal peptide diversity in bacilli

12.30 – 14.00 Lunch (+ BACIP meeting)

Session VI: Regulation and Metabolism Chair: Sabine Brantl 14.00 – 14.15 Airat Kayumov, Annette Heinrich, Kseniya Fedorova, Olga Ilinskaya, and Karl Forchhammer (Kazan State University of Architecture and Engineering, Kazan, Russia; and Eberhard-Karls-Universität Tübingen, Germany)

• • Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis

14.15 – 14.30 Jens Landmann, Ricarda Busse, Jörg Stülke, and Boris Görke (Georg-August-University of Göttingen, Germany)

• • Regulation of a glycolytic bypass by Crh, the homolog of the phospho-carrier protein HPr in Bacillus subtilis

14.30 – 14.45 Alexander K.W. Elsholz, Dan Oertel, Katrin Gronau, Dörte Becher, Kürsad Turgay, Michael Hecker, and Ulf Gerth (Ernst-Moritz-Arndt-University of Greifswald, Germany; and Freie Universität Berlin, Germany)

• • Arginine phosphorylation modulates ClpC activity in Bacillus subtilis

14.45 – 15.00 Ines Gruner, Anika March, Claudia Frädrich, and Elisabeth Härtig (Technical University of Braunschweig, Germany)

• • Regulation of anaerobic adaptation of Bacillus subtilis

15.00 – 15.15 Marcus Miethke, Florian Peuckert, and Mohamed A. Marahiel (Philipps University of Marburg, Germany)

• • Ferric siderophore uptake and intracellular iron release in Bacillus subtilis and Bacillus halodurans

15.15 – 15.30 S. Graf, and G. Unden (Universität Mainz, Germany)

• • The fumarate sensor DcuS of Geobacillus kaustophilus

15.30 – 15.45 Ken-ichi Yoshida (Kobe University, Kobe, Japan)

• • Natural interconversion of inositol stereoisomers in bacteria?

15.45 – 16.15 Coffee/Tea

Session VII: Cell wall and Sporulation Chair: Dörte Becher 16.15 – 16.30 Eric Botella, Sebastian Hübner, and Kevin M Devine (Trinity College Dublin, Ireland)

• • Cell envelope gene expression in phosphate limited Bacillus subtilis cells

16.30 – 16.45 Robyn T. Eijlander, and Oscar P. Kuipers (TI Food and Nutrition, Wageningen, the Netherlands; and University of Groningen, the Netherlands)

• • Studying Bacillus cereus sporulation and germination at the single cell level

16.45 – 17.00 Anthony J. Wilkinson (University of York, UK)

• • Structural and interaction studies of an intercellular zipper mediating engulfment during sporulation in B. subtilis

Session VIII: Gram-positive pathogens Chair: Imrich Barak 17.00 – 17.15 Simen M. Kristoffersen, Nicolas J. Tourasse, Timothy D. Read, Brian Desany, Anne-Brit Kolstø, and Ole Andreas Økstad (School of Pharmacy, University of Oslo, Norway; Emory University School of Medicine, Atlanta, GA, USA; and Life Sciences, A Roche Company, Branford, USA)

• • Eighteen interspersed DNA repeat elements in the Bacillus cereus group promote genome dynamics and plasticity, and may confer global and local effects on neighbour gene transcription

17.15 – 17.30 Susanne Pohl, Wang Yung Tu, Nigel Robinson, Kevin Waldron, and Colin Harwood (Newcastle University, Newcastle upon Tyne, United Kingdom)

• • The superoxide oxidative stress response of Bacillus anthracis

17.30 – 17.45 Vahid Farshchi Andisi, Anne de Jong, Oscar P. Kuipers, and Jetta J.E. Bijlsma (UMCG-University of Groningen, the Netherlands; and University of Groningen, the Netherlands)

• • Identification of a Streptococcus pneumoniae protein involved in resistance to oxidative stress

17.45 – 18.00 Annette Dreisbach, Magdalena M. van der Kooi-Pol, Andreas Otto, Katrin Gronau, Hendrik P. J. Bonarius, Hans Westra, Herman Groen, Dörte Becher, Michael Hecker, and Jan Maarten van Dijl (UMCG-University of Groningen, the Netherlands; Ernst-Moritz-Arndt-Universität, Greifswald, Germany; and IQ Therapeutics, Groningen, the Netherlands)

• • Profiling of global interactions between human serum proteins and the Staphylococcus aureus cell surface