proJ

168

glutamate 5-kinase

locus: BSU_18470

Molecular weight: 40.20 kDa

pI: 5.3

Protein length: 371 aa

Gene length: 1116 bp

function: osmoadaptive de novo production of proline

product: glutamate 5-kinase

essential: no

ec: 2.7.2.11

synonyms: proJ, yohA

List of homologs in different organisms, belongs to COG0263 (Galperin et al., 2021)

Gene

Coordinates: 2,015,733  2,016,848

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The protein

Catalyzed reaction/ biological activity

• ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)

Protein family

• glutamate 5-kinase family (with [protein|5592C5163CF024289B850916516E2C8C7AD43136|proB], according to UniProt)

[wiki|Domains]

• PUA domain (aa 280-356) (according to UniProt)

Structure

• [PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)
• [AF|O07509]

Paralogous protein(s)

• [protein|5592C5163CF024289B850916516E2C8C7AD43136|proB]

Biological materials

Mutant

• GP817 (spc) available in [wiki|Jörg Stülke]'s lab
• 1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]
• BKE18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG,  downstream forward: _UP4_TAATTTGCAGAAAAAAACCC
• BKK18470 ([gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTTTTCATGCTTGTATCAG,  downstream forward: _UP4_TAATTTGCAGAAAAAAACCC

References

Reviews

• 25367752

Original Publications

• 21296969,25344233,21784929,27766092,28752945,35593133