Difference between revisions of "ProJ"

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|style="background:#ABCDEF;" align="center"|'''Function''' || osmoadaptive de novo production of proline
 
|style="background:#ABCDEF;" align="center"|'''Function''' || osmoadaptive de novo production of proline
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU18470 proJ]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18470 proJ]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gltC]]'', ''[[proH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gltC]]'', ''[[proH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13740&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18470 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:proJ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:proJ_context.gif]]

Revision as of 12:58, 13 May 2013

  • Description: glutamate 5-kinase

Gene name proJ
Synonyms yohA
Essential no
Product glutamate 5-kinase
Function osmoadaptive de novo production of proline
Gene expression levels in SubtiExpress: proJ
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 40 kDa, 5.296
Gene length, protein length 1113 bp, 371 aa
Immediate neighbours gltC, proH
Sequences Protein DNA Advanced_DNA
Genetic context
ProJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ProJ expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU18470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s): ProB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • expressed under conditions of osmotic stress PubMed
    • induced by cold stress PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP817 (spc) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)