Difference between revisions of "ProJ"

From SubtiWiki
Jump to: navigation, search
Line 26: Line 26:
 
|colspan="2" | '''Genetic context''' <br/> [[Image:proJ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:proJ_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proJ_2015733_2016848_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:proJ_expression.png|500px]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
  
 
<br/><br/><br/><br/><br/><br/>
 
<br/><br/><br/><br/><br/><br/>

Revision as of 10:41, 19 April 2012

  • Description: glutamate 5-kinase

Gene name proJ
Synonyms yohA
Essential no
Product glutamate 5-kinase
Function osmoadaptive de novo production of proline
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 40 kDa, 5.296
Gene length, protein length 1113 bp, 371 aa
Immediate neighbours gltC, proH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ProJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ProJ expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU18470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s): ProB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • expressed under conditions of osmotic stress PubMed
    • induced by cold stress PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP817 (spc) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed