Difference between revisions of "ProJ"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP817 (spc) available in the [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 15:51, 7 February 2011

  • Description: glutamate 5-kinase

Gene name proJ
Synonyms yohA
Essential no
Product glutamate 5-kinase
Function biosynthesis of proline
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 40 kDa, 5.296
Gene length, protein length 1113 bp, 371 aa
Immediate neighbours gltC, proH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ProJ context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU18470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s): ProB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: expressed under conditions of osmotic stress
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP817 (spc) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References