Difference between revisions of "ProH"

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=The gene=
 
=The gene=

Revision as of 14:27, 22 June 2009

  • Description: pyrroline-5-carboxylate reductase

Gene name proH
Synonyms yoxE
Essential no
Product pyrroline-5-carboxylate reductase
Function biosynthesis of proline
Metabolic function and regulation of this protein in SubtiPathways:
Pro, Stress
MW, pI 31 kDa, 7.754
Gene length, protein length 891 bp, 297 aa
Immediate neighbours proJ, rtp
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ProH context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU18480

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)
  • Protein family: glutamate 5-kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: expressed under conditions of osmotic stress
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582] [WorldCat.org] [DOI] (P p)