Difference between revisions of "ProH"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proH_2016845_2017738_-1 proH] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proH_2016845_2017738_-1 proH] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|21784929}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21784929}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:53, 27 October 2014
- Description: pyrroline-5-carboxylate reductase
| Gene name | proH |
| Synonyms | yoxE |
| Essential | no |
| Product | pyrroline-5-carboxylate reductase |
| Function | osmoadaptive de novo production of proline |
| Gene expression levels in SubtiExpress: proH | |
| Metabolic function and regulation of this protein in SubtiPathways: proH | |
| MW, pI | 31 kDa, 7.754 |
| Gene length, protein length | 891 bp, 297 aa |
| Immediate neighbours | proJ, rtp |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU18480
Phenotypes of a mutant
Database entries
- BsubCyc: BSU18480
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)
- Protein family: glutamate 5-kinase family (according to Swiss-Prot)
- Paralogous protein(s): ProI
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU18480
- Structure:
- UniProt: P14383
- KEGG entry: [2]
- E.C. number: 1.5.1.2
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Adrienne Zaprasis, Monika Bleisteiner, Anne Kerres, Tamara Hoffmann, Erhard Bremer
Uptake of amino acids and their metabolic conversion into the compatible solute proline confers osmoprotection to Bacillus subtilis.
Appl Environ Microbiol: 2015, 81(1);250-9
[PubMed:25344233]
[WorldCat.org]
[DOI]
(I p)
Jeanette Brill, Tamara Hoffmann, Monika Bleisteiner, Erhard Bremer
Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity.
J Bacteriol: 2011, 193(19);5335-46
[PubMed:21784929]
[WorldCat.org]
[DOI]
(I p)
Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969]
[WorldCat.org]
[DOI]
(I p)
B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582]
[WorldCat.org]
[DOI]
(P p)
