PrkD

• Description: protein kinase D
  
  

Gene name	prkD
Synonyms	ybdM
Essential	no
Product	protein kinase D
Function	unknown
Gene expression levels in SubtiExpress: prkD
Interactions involving this protein in SubtInteract: PrkD
MW, pI	29 kDa, 6.359
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	ybzI, ybdN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphoproteins

The gene

Basic information

• Locus tag: BSU02030

Database entries

• BsubCyc: BSU02030

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylates DegS on Ser-76 (in vitro) PubMed

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: autophosphorylated on Thr-174 (in vitro) PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • AbrB-PrkD PubMed

• Localization:

Database entries

• BsubCyc: BSU02030

• Structure:

• UniProt: O31435

• KEGG entry: [2]

• E.C. number: 2.7.11.1

Expression and regulation

• Operon:

• Expression browser: prkD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP578 (aphA3), available in the Stülke lab

• Expression vector:
  • pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab

• lacZ fusion: pGP830 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ahasanul Kobir, Sandrine Poncet, Vladimir Bidnenko, Olivier Delumeau, Carsten Jers, Samira Zouhir, Rosa Grenha, Sylvie Nessler, Phillipe Noirot, Ivan Mijakovic
Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties.
Mol Microbiol: 2014, 92(5);1129-41
[PubMed:24731262] [WorldCat.org] [DOI] (I p)

Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic
Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
PLoS One: 2011, 6(2);e14653
[PubMed:21304896] [WorldCat.org] [DOI] (I e)

Nico Pietack, Dörte Becher, Sebastian R Schmidl, Milton H Saier, Michael Hecker, Fabian M Commichau, Jörg Stülke
In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism.
J Mol Microbiol Biotechnol: 2010, 18(3);129-40
[PubMed:20389117] [WorldCat.org] [DOI] (I p)

Edwige Madec, Agnieszka Laszkiewicz, Adam Iwanicki, Michal Obuchowski, Simone Séror
Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes.
Mol Microbiol: 2002, 46(2);571-86
[PubMed:12406230] [WorldCat.org] [DOI] (P p)