Difference between revisions of "PrkD"

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Revision as of 12:26, 16 May 2013

Gene name prkD
Synonyms ybdM
Essential no
Product protein kinase D
Function unknown
Gene expression levels in SubtiExpress: prkD
MW, pI 29 kDa, 6.359
Gene length, protein length 768 bp, 256 aa
Immediate neighbours ybzI, ybdN
Sequences Protein DNA DNA_with_flanks
Genetic context
YbdM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrkD expression.png
























Categories containing this gene/protein

protein modification, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU02030

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
    • phosphorylates DegS on Ser-76 (in vitro) PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: autophosphorylated on Thr-174 (in vitro) PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP578 (aphA3), available in the Stülke lab
  • Expression vector:
    • pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab
  • lacZ fusion: pGP830 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic
Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
PLoS One: 2011, 6(2);e14653
[PubMed:21304896] [WorldCat.org] [DOI] (I e)

Nico Pietack, Dörte Becher, Sebastian R Schmidl, Milton H Saier, Michael Hecker, Fabian M Commichau, Jörg Stülke
In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism.
J Mol Microbiol Biotechnol: 2010, 18(3);129-40
[PubMed:20389117] [WorldCat.org] [DOI] (I p)

Edwige Madec, Agnieszka Laszkiewicz, Adam Iwanicki, Michal Obuchowski, Simone Séror
Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes.
Mol Microbiol: 2002, 46(2);571-86
[PubMed:12406230] [WorldCat.org] [DOI] (P p)