prkC

prkC
168

protein kinase C, fine-tuning of cell growth rates in response to Lipid II in different nutrient conditions, induces [category|SW.4.2.4|Germination] of spores in response to DAP-type, and not to Lys-type cell wall muropeptides, stimulates [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] activity

locus
BSU_15770
Molecular weight
71.69 kDa
pI
4.83
Protein length
Gene length
function
regulation of growth and germination in response to muropeptides and Lipid II
product
protein kinase
essential
no
ec
2.7.11.1
synonyms
prkC, yloP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2815 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,651,142  1,653,088
Phenotypes of a mutant
unable to germinate in response to muropeptides [Pubmed|18984160]
reduced growth at high salt [Pubmed|25845974]
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The protein
Catalyzed reaction/ biological activity
phosphorylation of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] on Thr-101 to stimulate its activity [Pubmed|26102633]
phosphorylates PrkA on Thr-217 [pubmed|36041628]
phosphorylation of RodZ in response to lipid II abundance [pubmed|36797487]
Protein family
[wiki|protein kinase superfamily] (according to UniProt)
[wiki|Ser/Thr protein kinase family] (according to UniProt)
[wiki|Domains]
contains three C-terminal [wiki|PASTA domain]s (aa 356-424, 425-492, 493-559) (binds muropeptides) [Pubmed|18984160]
[wiki|Protein kinase domain] (aa 11-271) (according to UniProt)
Structure
[PDB|4EQM] (intracellular domain of the ''Staphylococcus aureus'' enzyme, 51% identity) [Pubmed|22701750]
[PDB|3PY9] (entire extra-cellular region of PrkC from ''Staphylococcus aureus'')  [Pubmed|21208192]
[PDB|4X3F] (intracellular domain of the ''Mycobacterium tuberculosis'' enzyme, 36% identity, 68% similarity) [Pubmed|25586004]
[AF|O34507]
Modification
phosphorylation on Thr-290 [Pubmed|17218307], autophosphorylation on multiple threonine residues [Pubmed|12842463,20389117]
Effectors of protein activity
activated by muropeptides [Pubmed|18984160]
lipid II [pubmed|36797487]
[protein|C22F7704DC9D36D96FE089ADCEA546D7A3FB3739|gpsB], [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|divIVA], and [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA] are required for stimulation of [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC] activity [Pubmed|25845974]
[wiki|Localization]
inner spore membrane [Pubmed|18984160]
membrane [Pubmed|12406230]
division septum [Pubmed|25845974]
Expression and Regulation
Operons
genes
[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
description
[Pubmed|22383849]
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[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]→[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]

2024-03-16 08:37:58

Jstuelk

87

f45e3810755c3fba238243b21c1c17e072c8fe47

14D2827FA6BD60210DFADC1BB1FFF0C98DC768FA

Biological materials
Mutant
MGNA-B134 (yloP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1133 NBRP B. subtilis, Japan]
GP576 (spc), OMG302 (aphA3), available in [wiki|Jörg Stülke]'s lab
1A820 ( ''prkC''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A820&Search=1A820 BGSC]
1A963 (no resistance), [Pubmed|12399479], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A963&Search=1A963 BGSC]
1A964 (no resistance), [Pubmed|12399479], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A964&Search=1A964 BGSC]
BKE15770 ([gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15770 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATTAGCACTGATCTTCACC,  downstream forward: _UP4_GATGAATAACAAGGAGGGAA
BKK15770 ([gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15770 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATTAGCACTGATCTTCACC,  downstream forward: _UP4_GATGAATAACAAGGAGGGAA
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP832, available in [wiki|Jörg Stülke]'s lab
for expression/ purification of the kinase domain from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], in [wiki|pGP380]: pGP849, available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP1001,  available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172]: pGP825,  available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
lacZ fusion
pGP829 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
20972452,21372323,26429880,27148245,31405912
Phosphorylation of PrkC
12842463,20563625
Phsiological role of PrkC
12399479,12406230,19246764,12842463,18984160,26102633,31723135,36797487
Structure/ biochemistry of PrkC
21208192,22111897,23793375,25668224,25586004,22701750
Expression/ localization of PrkC
16025310,24752279,26731423,29374241
Targets of PrkC-dependent phosphorylation
19246764,20070526,20389117,24390483,24731262,25012659,25278935,25845974,26102633,30478337,36041628

23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA

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Time of last update: 2024-03-28 14:58:48

Author of last update: Jstuelk