PrfB

• Description: peptide chain release factor 2
  
  

Gene name	prfB
Synonyms
Essential	yes PubMed
Product	peptide chain release factor 2
Function	translation
Gene expression levels in SubtiExpress: prfB
Metabolic function and regulation of this protein in SubtiPathways: PrfB
MW, pI	41 kDa, 4.85
Gene length, protein length	1099 bp, 366 aa
Immediate neighbours	yvjA, secA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU35290

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU35290

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA

• Protein family: prokaryotic/mitochondrial release factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35290

• Structure:

• UniProt: P28367

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: secA-prfB PubMed

• Expression browser: prfB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 662 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1710 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3037 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1746 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1959 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Johan Sund, Martin Andér, Johan Aqvist
Principles of stop-codon reading on the ribosome.
Nature: 2010, 465(7300);947-50
[PubMed:20512119] [WorldCat.org] [DOI] (I p)

M Herbort, M Klein, E H Manting, A J Driessen, R Freudl
Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase.
J Bacteriol: 1999, 181(2);493-500
[PubMed:9882663] [WorldCat.org] [DOI] (P p)