PrfB

• Description: peptide chain release factor 2
  
  

Gene name	prfB
Synonyms
Essential	yes PubMed
Product	peptide chain release factor 2
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	41 kDa, 4.85
Gene length, protein length	1099 bp, 366 aa
Immediate neighbours	yvjA, secA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU35290

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA

• Protein family: prokaryotic/mitochondrial release factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P28367

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: secA-prfB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Johan Sund, Martin Andér, Johan Aqvist
Principles of stop-codon reading on the ribosome.
Nature: 2010, 465(7300);947-50
[PubMed:20512119] [WorldCat.org] [DOI] (I p)

M Herbort, M Klein, E H Manting, A J Driessen, R Freudl
Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase.
J Bacteriol: 1999, 181(2);493-500
[PubMed:9882663] [WorldCat.org] [DOI] (P p)