ytdI

168

inorganic polyphosphate/ATP-NAD kinase

locus: BSU_29540

Molecular weight: 30.10 kDa

pI: 6.49

Protein length: 267 aa

Gene length: 804 bp

function: generation of NADP from NAD

product: inorganic polyphosphate/ATP-NAD kinase

essential: no

ec: 2.7.1.23

synonyms: ytdI, ppnKB, nadK2

List of homologs in different organisms, belongs to COG0061 (Galperin et al., 2021)

Gene

Coordinates: 3,021,233 → 3,022,036

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The protein

Catalyzed reaction/ biological activity

• ATP + NAD⁺ --> ADP + H⁺ + NADP⁺ (according to UniProt)

Protein family

• NAD kinase family (with [protein|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF], according to UniProt)

Structure

• [PDB|1YT5] (from ''Thermotoga maritima'')\t[Pubmed|16511117]
• [AF|O34934]

Paralogous protein(s)

• [protein|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]

  description: [Pubmed|21815947]

  additional information

  • half-life of the mRNA: 8.4 min, in a [gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS] mutant 15 min, in a [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|rny] mutant 24 min [PubMed|25643072]

Other regulations

• [protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]: translation inhibition, inhibition of translation and initiation of RNA degradation by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|rny] and [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc] [Pubmed|25643072] translation is inhibited by binding of the [protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS] RNA to the mRNA [Pubmed|25643072]

Biological materials

Mutant

• MGNA-A109 (ytdI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/109 NBRP B. subtilis, Japan]
• BKE29540 (Δ[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCCCATTTCCCCTTTG,  downstream forward: _UP4_TAGAAAATAAAAGGACAGGC
• BKK29540 (Δ[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCCCATTTCCCCTTTG,  downstream forward: _UP4_TAGAAAATAAAAGGACAGGC

References

• 16511117,25643072,21815947,36815589