PolX

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  • Description: DNA polymerase X, involved in DNA repair

Gene name polX
Synonyms yshC
Essential
Product DNA polymerase X
Function involved in base excision repair
MW, pI 63 kDa, 5.308
Gene length, protein length 1710 bp, 570 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
YshC context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: template-dependent DNA polymerase, fills single nucleotide gaps PubMed, has intrinsic 3'-5' exonuclease activity for resecting unannealed 3'-termini in gapped DNA substrates PubMed
  • Protein family: DNA polymerase X family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: monomeric PubMed
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Margarita Salas, Madrid, Spain link

Your additional remarks

References

  1. Banos et al. (2008) Characterization of a Bacillus subtilis 64 kDa DNA polymerase X potentially involved in DNA repair. J. Mol. Biol. 384: 1019-1028. PubMed
  2. Banos et al. (2008) Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase. Nucl. Acids Res. 36: 5736-5749. PubMed