Difference between revisions of "PolA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA replication | |style="background:#ABCDEF;" align="center"|'''Function''' || DNA replication | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29090 polA] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 98 kDa, 4.916 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 98 kDa, 4.916 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutM]]'', ''[[phoR]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutM]]'', ''[[phoR]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29090 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:polA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:polA_context.gif]] | ||
Revision as of 13:34, 13 May 2013
- Description: DNA polymerase I, required for repair of UV- or MMS-induced DNA damage
| Gene name | polA |
| Synonyms | |
| Essential | no |
| Product | DNA polymerase I |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: polA | |
| MW, pI | 98 kDa, 4.916 |
| Gene length, protein length | 2640 bp, 880 aa |
| Immediate neighbours | mutM, phoR |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29090
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity PubMed
- Protein family: DNA polymerase type-A family (according to Swiss-Prot)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1XWL (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)
- UniProt: O34996
- KEGG entry: [2]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985]
[WorldCat.org]
[DOI]
(I p)
Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254]
[WorldCat.org]
[DOI]
(P p)
Stéphane Duigou, S Dusko Ehrlich, Philippe Noirot, Marie-Françoise Noirot-Gros
DNA polymerase I acts in translesion synthesis mediated by the Y-polymerases in Bacillus subtilis.
Mol Microbiol: 2005, 57(3);678-90
[PubMed:16045613]
[WorldCat.org]
[DOI]
(P p)
