PncB

• Description: putative nicotinate phosphoribosyltransferase
  
  

Gene name	pncB
Synonyms	yueK
Essential	yes PubMed
Product	putative nicotinate phosphoribosyltransferase
Function	unknown
Gene expression levels in SubtiExpress: pncB
Metabolic function and regulation of this protein in SubtiPathways: PncB
MW, pI	56 kDa, 5.089
Gene length, protein length	1470 bp, 490 aa
Immediate neighbours	pdeH, pncA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

essential genes, poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU31750

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU31750

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: NAPRTase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU31750

• Structure:

• UniProt: O32090

• KEGG entry: [3]

• E.C. number: 2.4.2.11

Expression and regulation

• Operon: pncB PubMed

• Expression browser: pncB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 526 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1729 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)