PncB

From SubtiWiki
Revision as of 15:31, 7 August 2012 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: putative nicotinate phosphoribosyltransferase

Gene name pncB
Synonyms yueK
Essential yes PubMed
Product putative nicotinate phosphoribosyltransferase
Function unknown
Gene expression levels in SubtiExpress: pncB
MW, pI 56 kDa, 5.089
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours yuxH, pncA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YueK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PncB expression.png
























Categories containing this gene/protein

essential genes, poorly characterized/ putative enzymes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31750

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family: NAPRTase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)