PnbA

• Description: para-nitrobenzyl esterase
  
  

Gene name	pnbA
Synonyms	estB
Essential	no
Product	para-nitrobenzyl esterase
Function	lipid degradation
Gene expression levels in SubtiExpress: pnbA
MW, pI	53 kDa, 4.776
Gene length, protein length	1467 bp, 489 aa
Immediate neighbours	slrR, padC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids, phosphoproteins

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU34390

Database entries

• BsubCyc: BSU34390

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Triacylglycerol + H₂O = diacylglycerol + a carboxylate (according to Swiss-Prot)

• Protein family: type-B carboxylesterase/lipase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-189 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU34390

• Structure: 1C7J PubMed

• UniProt: P37967

• KEGG entry: [2]

• E.C. number: 3.1.1.3 3.1.1.1]

Expression and regulation

• Operon:

• Expression browser: pnbA PubMed

• Sigma factor:

• Regulation:
  • repressed during logarithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 745 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 382 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 676 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 446 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 891 PubMed

Biological materials

• Mutant:
  • GP951 (pnbA::cat), available in Stülke lab
  • GP955 (slrR-pnbA::cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: