PnbA

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  • Description: para-nitrobenzyl esterase

Gene name pnbA
Synonyms estB
Essential no
Product para-nitrobenzyl esterase
Function lipid degradation
Gene expression levels in SubtiExpress: pnbA
MW, pI 53 kDa, 4.776
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours slrR, padC
Sequences Protein DNA DNA_with_flanks
Genetic context
PnbA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PnbA expression.png
























Categories containing this gene/protein

utilization of lipids, phosphoproteins

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

  • Locus tag: BSU34390

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate (according to Swiss-Prot)
  • Protein family: type-B carboxylesterase/lipase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-189 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • repressed during logarithmic growth (AbrB) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 745 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 382 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 676 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 446 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 891 PubMed

Biological materials

  • Mutant:
    • GP951 (pnbA::cat), available in Stülke lab
    • GP955 (slrR-pnbA::cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Xiaozhen Yu, Sara C Sigler, Delwar Hossain, Monika Wierdl, Steven R Gwaltney, Philip M Potter, Randy M Wadkins
Global and local molecular dynamics of a bacterial carboxylesterase provide insight into its catalytic mechanism.
J Mol Model: 2012, 18(6);2869-83
[PubMed:22127613] [WorldCat.org] [DOI] (I p)

Doris Ribitsch, Sonja Heumann, Eva Trotscha, Enrique Herrero Acero, Katrin Greimel, Regina Leber, Ruth Birner-Gruenberger, Sigrid Deller, Inge Eiteljoerg, Peter Remler, Thomas Weber, Petra Siegert, Karl-Heinz Maurer, Ilaria Donelli, Giuliano Freddi, Helmut Schwab, Georg M Guebitz
Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis.
Biotechnol Prog: 2011, 27(4);951-60
[PubMed:21574267] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

B Spiller, A Gershenson, F H Arnold, R C Stevens
A structural view of evolutionary divergence.
Proc Natl Acad Sci U S A: 1999, 96(22);12305-10
[PubMed:10535917] [WorldCat.org] [DOI] (P p)

J Zock, C Cantwell, J Swartling, R Hodges, T Pohl, K Sutton, P Rosteck, D McGilvray, S Queener
The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli.
Gene: 1994, 151(1-2);37-43
[PubMed:7828905] [WorldCat.org] [DOI] (P p)