pnbA

168

para-nitrobenzyl esterase

locus: BSU_34390

Molecular weight: 53.82 kDa

pI: 4.78

Protein length: 489 aa

Gene length: 1470 bp

function: lipid degradation

product: para-nitrobenzyl esterase

essential: no

ec: 3.1.1.3

synonyms: pnbA, estB

List of homologs in different organisms, belongs to COG2272 (Galperin et al., 2021)

Gene

Coordinates: 3,530,635  3,532,104

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The protein

Protein family

• type-B carboxylesterase/lipase family (single member, according to UniProt)

Structure

• [PDB|1C7J]\t[Pubmed|10535917]
• [AF|P37967]

Modification

• phosphorylation on Ser-189 [Pubmed|17218307]

Biological materials

Mutant

• GP951 (''pnbA''::''cat''), available in [wiki|Jörg Stülke]'s lab
• GP955 (''[gene|920F91E748EE079FF864011D9052B073567C41E4|slrR]''-''pnbA''::''cat''), available in [wiki|Jörg Stülke]'s lab
• BKE34390 ([gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34390 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT,  downstream forward: _UP4_TAAATATGGGGAAAACAGGA
• BKK34390 ([gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34390 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT,  downstream forward: _UP4_TAAATATGGGGAAAACAGGA

References

• 7828905,21574267,17218307,10535917,22127613,25077141,37021103,38235660