Difference between revisions of "PlsY"

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(Extended information on the protein)
(Biological materials)
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:'''  
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* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab {{PubMed|19282621}}
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 07:45, 28 July 2009

  • Description: acylphosphate:glycerol-phosphate acyltransferase

Gene name plsY
Synonyms yneS
Essential yes PubMed
Product acylphosphate:glycerol-phosphate acyltransferase
Function biosynthesis of phospholipids
MW, pI 20 kDa, 10.408
Gene length, protein length 579 bp, 193 aa
Immediate neighbours yneR, yneT
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YneS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU18070

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0078 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yoshinori Hara, Masahide Seki, Satoshi Matsuoka, Hiroshi Hara, Atsushi Yamashita, Kouji Matsumoto
Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis.
Genes Genet Syst: 2008, 83(6);433-42
[PubMed:19282621] [WorldCat.org] [DOI] (P p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)