plsX

168

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO₄)

locus: BSU_15890

Molecular weight: 35.61 kDa

pI: 5.49

Protein length: 333 aa

Gene length: 1002 bp

function: biosynthesis of phospholipids

product: acyl-acyl carrier protein (ACP):phosphate acyltransferase

essential: yes

ec: 2.3.1.n2

synonyms: plsX, ylpD

List of homologs in different organisms, belongs to COG0416 (Galperin et al., 2021)

Gene

Coordinates: 1,662,547  1,663,548

Phenotypes of a mutant

• essential [Pubmed|24224907,12682299]
• depletion results in sporulation deficiency (via [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]) [Pubmed|22111992]
• a ts mutant forms an abberant Z-ring and has a [wiki|cell division] defect [Pubmed|24224907]
• depletion results in accumulation of malonyl-CoA and of long-chain-ACPs [pubmed|32671874]

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The protein

Catalyzed reaction/ biological activity

• catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO₄)
• fatty acyl-[ACP] + phosphate --> acyl phosphate + holo-[ACP] (according to UniProt)

Protein family

• plsX family (single member, according to UniProt)

Structure

• [PDB|1VI1] [Pubmed|16021622]
• [PDB|6A1K] [pubmed|31453380]
• [AF|P71018]

[wiki|Localization]

• localizes to regions of increased fluidity in the membrane (via a short amphipathic α-peptide) [pubmed|31453380,31925177]
• uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]
• in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]

Biological materials

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in Matsumoto lab [Pubmed|19282621]

References

• 12737802,19282621,17645809,17557823,8759840,16932747,19850612,16091051,21383089,22111992,24224907,26816052,16021622,27791134,31453380,31925177,31919098,32180316,32671874