Difference between revisions of "PlsX"

From SubtiWiki
Jump to: navigation, search
(Undo revision 120366 by Adr (Talk))
Line 13: Line 13:
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of phospholipids
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_syn.html Fatty acid and phospholipid biosynthesis]'''
 
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.492   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.492   

Revision as of 10:15, 16 February 2010

  • Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase

Gene name plsX
Synonyms ylpD
Essential yes PubMed
Product acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function biosynthesis of phospholipids
MW, pI 35 kDa, 5.492
Gene length, protein length 999 bp, 333 aa
Immediate neighbours fapR, fabD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PlsX context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15890

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: plsX family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
    • expressed at high cell density (ComA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mariano A Martinez, Diego de Mendoza, Gustavo E Schujman
Transcriptional and functional characterization of the gene encoding acyl carrier protein in Bacillus subtilis.
Microbiology (Reading): 2010, 156(Pt 2);484-495
[PubMed:19850612] [WorldCat.org] [DOI] (I p)

Yoshinori Hara, Masahide Seki, Satoshi Matsuoka, Hiroshi Hara, Atsushi Yamashita, Kouji Matsumoto
Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis.
Genes Genet Syst: 2008, 83(6);433-42
[PubMed:19282621] [WorldCat.org] [DOI] (P p)

Mika Yoshimura, Taku Oshima, Naotake Ogasawara
Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli.
BMC Microbiol: 2007, 7;69
[PubMed:17645809] [WorldCat.org] [DOI] (I e)

Luciana Paoletti, Ying-Jie Lu, Gustavo E Schujman, Diego de Mendoza, Charles O Rock
Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis.
J Bacteriol: 2007, 189(16);5816-24
[PubMed:17557823] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Marcelo Guerin, Alejandro Buschiazzo, Francis Schaeffer, Leticia I Llarrull, Georgina Reh, Alejandro J Vila, Pedro M Alzari, Diego de Mendoza
Structural basis of lipid biosynthesis regulation in Gram-positive bacteria.
EMBO J: 2006, 25(17);4074-83
[PubMed:16932747] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Luciana Paoletti, Alan D Grossman, Diego de Mendoza
FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis.
Dev Cell: 2003, 4(5);663-72
[PubMed:12737802] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.
J Bacteriol: 1996, 178(16);4794-800
[PubMed:8759840] [WorldCat.org] [DOI] (P p)