Difference between revisions of "PlsX"

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(Biological materials)
(Biological materials)
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab {{PubMed|19282621}}
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* '''two-hybrid system:'''  ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab {{PubMed|19282621}}
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 07:45, 28 July 2009

  • Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase

Gene name plsX
Synonyms ylpD
Essential yes PubMed
Product acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function biosynthesis of phospholipids
MW, pI 35 kDa, 5.492
Gene length, protein length 999 bp, 333 aa
Immediate neighbours fapR, fabD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PlsX context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15890

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: plsX family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed unless the cells experience a lack of malonyl-CoA (FapR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yoshinori Hara, Masahide Seki, Satoshi Matsuoka, Hiroshi Hara, Atsushi Yamashita, Kouji Matsumoto
Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis.
Genes Genet Syst: 2008, 83(6);433-42
[PubMed:19282621] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Luciana Paoletti, Alan D Grossman, Diego de Mendoza
FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis.
Dev Cell: 2003, 4(5);663-72
[PubMed:12737802] [WorldCat.org] [DOI] (P p)