pksF

168

involved in polyketide synthesis

locus: BSU_17140

Molecular weight: 44.97 kDa

pI: 5.37

Protein length: 416 aa

Gene length: 1248 bp

function: polyketide synthesis

product: unknown

essential: no

synonyms: pksF

List of homologs in different organisms, belongs to COG0304 (Galperin et al., 2021)

Gene

Coordinates: 1,788,695 → 1,789,942

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The protein

Catalyzed reaction/ biological activity

• H⁺ + malonyl-[ACP] --> acetyl-[ACP] + CO₂ (according to UniProt)

Protein family

• [wiki|thiolase-like superfamily] (according to UniProt)

Structure

• [PDB|4LS5] ([protein|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF], 30% identity) [pubmed|24641521]
• [AF|P40804]

Biological materials

Mutant

• BKE17140 (Δ[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACGCCTACACCGGTCACCA,  downstream forward: _UP4_GAGAAATGTGGAGGCGAATC
• BKK17140 (Δ[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACGCCTACACCGGTCACCA,  downstream forward: _UP4_GAGAAATGTGGAGGCGAATC

References

• 22383849,24187085,27766092,24641521