pksE

168

involved in polyketide synthesis

locus: BSU_17120

Molecular weight: 85.61 kDa

pI: 7.38

Protein length: 767 aa

Gene length: 2304 bp

function: polyketide synthesis

product: unknown

essential: no

synonyms: pksE

List of homologs in different organisms, belongs to COG2070 (Galperin et al., 2021)

Gene

Coordinates: 1,786,104 → 1,788,407

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The protein

Catalyzed reaction/ biological activity

• holo-[ACP] + malonyl-CoA --> CoA + malonyl-[ACP] (according to UniProt)

Protein family

• fabD family (with [protein|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC] and [protein|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD], according to UniProt)

Structure

• [PDB|5DZ7] (N-terminal domain, aa 1 ... 288)
• [PDB|4CW5] (C-terminal domain, from B. amyloliquefaciens, corresponds to aa 315 ... 753, 59% identity) [pubmed|25456814]
• [AF|O34787]

Biological materials

Mutant

• BKE17120 (Δ[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCCCTGGAAAGACATATG,  downstream forward: _UP4_TGAATATTATGAAAATATAC
• BKK17120 (Δ[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCCCTGGAAAGACATATG,  downstream forward: _UP4_TGAATATTATGAAAATATAC

References

• 12850135,17190806,22383849,24187085,25456814