pksD

168

involved in polyketide synthesis, similar to loading acyltransferase

locus: BSU_17110

Molecular weight: 33.91 kDa

pI: 5.78

Protein length: 324 aa

Gene length: 975 bp

function: polyketide synthesis

product: unknown

essential: no

synonyms: pksD

List of homologs in different organisms, belongs to COG3321 (Galperin et al., 2021)

Gene

Coordinates: 1,785,133 → 1,786,107

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The protein

Structure

• [PDB|4RL1] (from Streptomyces avermitilis, 26% identity) [pubmed|25581064]
• [AF|O34877]

Biological materials

Mutant

• BKE17110 (Δ[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATAACCTTCTTTT,  downstream forward: _UP4_ACACCAGAAAGGAAGTTTAC
• BKK17110 (Δ[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATAACCTTCTTTT,  downstream forward: _UP4_ACACCAGAAAGGAAGTTTAC

References

• 22383849,24187085,25581064