Phy

• Description: phytase
  
  

Gene name	phy
Synonyms	yodV, yzxA
Essential	no
Product	3-phytase
Function	untilization of inositol hexakisphosphate (phytate)
MW, pI	41 kDa, 4.936
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	cgeB, yodU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Myo-inositol hexakisphosphate + H₂O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate (according to Swiss-Prot)

• Protein family: msrA Met sulfoxide reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 1QLG (enzyme from B. amyloliquefaciens, 72% identity)

• UniProt: P42094

• KEGG entry: [3]

• E.C. number: 3.1.3.8

Expression and regulation

• Operon: phy PubMed

• Sigma factor: none PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • the gene is not expressed in B. subtilis due to the absene of a functional promoter PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Martha Guerrero-Olazarán, Lilí Rodríguez-Blanco, J Gerardo Carreon-Treviño, Juan A Gallegos-López, José M Viader-Salvadó
Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme.
Appl Environ Microbiol: 2010, 76(16);5601-8
[PubMed:20601512] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Oliwia Makarewicz, Sarah Dubrac, Tarek Msadek, Rainer Borriss
Dual role of the PhoP approximately P response regulator: Bacillus amyloliquefaciens FZB45 phytase gene transcription is directed by positive and negative interactions with the phyC promoter.
J Bacteriol: 2006, 188(19);6953-65
[PubMed:16980498] [WorldCat.org] [DOI] (P p)

N C Ha, B C Oh, S Shin, H J Kim, T K Oh, Y O Kim, K Y Choi, B H Oh
Crystal structures of a novel, thermostable phytase in partially and fully calcium-loaded states.
Nat Struct Biol: 2000, 7(2);147-53
[PubMed:10655618] [WorldCat.org] [DOI] (P p)

S Roels, R Losick
Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis.
J Bacteriol: 1995, 177(21);6263-75
[PubMed:7592393] [WorldCat.org] [DOI] (P p)