PhrE

• Description: response regulator aspartate phosphatase (RapE) regulator, control of the phosphorelay
  
  

Gene name	phrE
Synonyms
Essential	no
Product	phosphatase (RapE) regulator
Function	control of sporulation initiation
Gene expression levels in SubtiExpress: phrE
Interactions involving this protein in SubtInteract: PhrE
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	4 kDa, 8.863
Gene length, protein length	132 bp, 44 aa
Immediate neighbours	rapE, yqzI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphorelay, quorum sensing, Skin element, short peptides

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, CodY regulon, ComA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU25840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O32025

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapE-phrE PubMed
  • phrE PubMed

• Expression browser: phrE PubMed

• Sigma factor:
  • phrE: SigA PubMed, SigH PubMed

• Regulation: repressed by glucose (10-fold) (CcpA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • ComA: transcription activation PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)

M Jiang, R Grau, M Perego
Differential processing of propeptide inhibitors of Rap phosphatases in Bacillus subtilis.
J Bacteriol: 2000, 182(2);303-10
[PubMed:10629174] [WorldCat.org] [DOI] (P p)