phoD

168

phosphodiesterase/alkaline phosphatase, degrades wall teichoic acid during phosphate starvation

locus: BSU_02620

Molecular weight: 62.66 kDa

pI: 8.39

Protein length: 583 aa

Gene length: 1752 bp

function: aquisition of phosphate upon phosphoate starvation, degradation of wall teichoic acid during phosphate starvation

product: phosphodiesterase/alkaline phosphatase

essential: no

ec: 3.1.3.1

synonyms: phoD, ycbS

List of homologs in different organisms, belongs to COG3540 (Galperin et al., 2021)

Gene

Coordinates: 284,011  285,762

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The protein

Catalyzed reaction/ biological activity

• endohydrolytic activity on wall teichoic acid, acts in concert with [protein|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ] [Pubmed|27780866]
• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• PhoD family (single member, according to UniProt)

[wiki|Cofactors]

• one Fe³⁺ and two Ca²⁺ [Pubmed|25217636]

Structure

• [PDB|2YEQ] [Pubmed|25217636]
• [AF|P42251]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862], secreted by the [protein|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD] complex  [Pubmed|19395490]

Biological materials

Mutant

• BKE02620 ([gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC,  downstream forward: _UP4_ATCACGAATTAAGGAGTGGA
• BKK02620 ([gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC,  downstream forward: _UP4_ATCACGAATTAAGGAGTGGA

labs

• [wiki|Oscar Kuipers], University of Groningen, The Netherlands
• [http://molgen.biol.rug.nl/molgen/index.php Homepage]

References

Reviews

• 24140208

Original Publications

• 8760916,10913081,12867413,10094677,12218047,10556724,19180538,10094677,18957862,19383693,19395490,22960285,22383849,23180473,25217636,25666134,27118079,27780866